Optimization for Enhanced Biodecolourization of Textile Dye Reactive Violet 5R by Pseudomonas Aeruginosa NGKCTS

Abstract

Soil samples collected from dye contaminated sites of Vatva, Gujarat, India were studied for the screening and isolation of organisms capable of decolourizing textile dyes. The most efficient isolate, which exhibited 95+1% decolourization of Reactive Violet 5R (RV5) within 8 h, was identified as Pseudomonas aeruginosa. The isolate was capable of decolourizing more than 95% of RVS over a wide pH range from 4.0 to 10.5 and temperature ranging from 25 to 50 °C. The culture could decolourize more than 95% of the dye under static conditions in the presence of glucose, peptone, yeast extract and starch. Interestingly, the culture showed more than 93% decolourization in the presence of 20 g/L NaCl concentration, and more than 79% decolourization in the presence of 100 g/L NaCl concentration. Addition of RV5, in each step, in ongoing dye decolourization flask, gave more than 92% decolourization within 0.75 h. The culture showed 95% decolourization of the dye at a concentration of 1000 mg/L within 28 h and could also decolourize 9 different reactive dyes at a concentration of 300 mg/L. The degradation of RV5 was confirmed by HPTLC. The culture also removed 73% copper present in RVS molecule.