Bacterial B-Galactosidase production

Abstract

12 bacterial isolates were isolated from sewage sample, compost and dairy soil sample and assessed for their B-galactosidase production. The most efficient producer RbNb, was identified as Klebsilla pneumonia. The characterization of the enzyme and optimization of the production medium were carried out for the maximum production and activity of B-galactosidase. Maximum production of the enzyme was observed when the medium was incubated for 48h at the temperature 37°C and maintained at pH 8. Use of lactose (1%) and yeast extract (2%) increase the enzyme production. The enzyme was precipitated by ammonium sulphate then fraction and Native - PAGE electrophoresis were carried out. The obtained molecular weight was - 23kDa and optimum enzymatic activity was found at 35°C and pH 7. Further enzyme activity was enhanced in the presence of MnCl2 and COCI2 at 10mM concentration From the result it is concluded that Klebsilla pneumonia is the most active isolate giving intracellular Bgalactosidase which is stable at 30-35°C for 150min andpH 7for 120mm